オンラインカジノならカジノレオ（CasinoLeo)

Academic Research Staff Details of a Researcher Home Japanese このページはJavascriptを使用しています。すべての機能を使用するためにはJavascript を有効にする必要があります。 Updated on 2023/10/17 Keyword・Affiliation・Field・Alphabetical Search Personnel Information 　 Profile 　 From Graduate School 　 Degree 　 Employment Record in Research Research Activity 　 Papers Updated on 2023/10/17 YAMAMOTO Kumiko Assistant Professor Title Assistant Professor External Link To the head of this page.▲ From Graduate School 【 display / non-display 】 To the head of this page.▲ From Graduate School 【 display / non-display 】 Hokkaido University   Graduate School, Division of Veterinary Medicine   Doctor Course   Completed 2016.04 - 2020.03 　 More details Country：Japan Hokkaido University of Education   Graduate School, Division of Education   Master Course   Completed 2014.04 - 2016.03 　 More details Country：Japan To the head of this page.▲ Degree 【 display / non-display 】 To the head of this page.▲ Degree 【 display / non-display 】 博士（獣医学） ( 2020.03   北海道大学 ) 修士（教育学） ( 2016.03   北海道教育大学 ) To the head of this page.▲ Employment Record in Research 【 display / non-display 】 To the head of this page.▲ Employment Record in Research 【 display / non-display 】 Tokyo University of Agriculture   Faculty of Bio-Industry   Department of Food, Aroma and Cosmetic Chemistry   Assistant Professor 2020.04 To the head of this page.▲ Papers 【 display / non-display 】 To the head of this page.▲ Papers 【 display / non-display 】 Electron Paramagnetic Resonance Implemented with Multiple Harmonic Detections Successfully Maps Extracellular pH In Vivo Ririko Nakaoka, Kazuhiro Kato, Kumiko Yamamoto, Hironobu Yasui, Shingo Matsumoto, Igor A. Kirilyuk, Valery V. Khramtsov, Osamu Inanami, Hiroshi Hirata Analytical Chemistry   2023.02 　More details Publishing type：Research paper (scientific journal)   Publisher：American Chemical Society (ACS)   DOI： 10.1021/acs.analchem.2c03194 researchmap Metformin preferentially enhances the radio-sensitivity of cancer stem-like cells with highly mitochondrial respiration ability in HMPOS. International journal Tatsuya Deguchi, Kenji Hosoya, Shango Kim, Yusuke Murase, Kumiko Yamamoto, Tomoki Bo, Hironobu Yasui, Osamu Inanami, Mahiro Okumura Molecular therapy oncolytics   22   143 - 151   2021.09 　More details Language：English   Publishing type：Research paper (scientific journal)   Metformin has many anti-cancer effects, alone or in combination with radiation. However, the mechanism underlying its radio-sensitized effect is still unclear, especially for cancer stem-like cells (CSCs). Here, the radio-sensitized effect of metformin was investigated, and its mechanism was revealed in CSCs derived from canine osteosarcoma cell line (HMPOS), a canine osteosarcoma cell line. Spheroid cells (SCs) were used as CSCs-rich cells derived from sphere formation, and SCs were compared with normal adherent culture cells (ACs). The radio-sensitizing effect of metformin using clonogenic assay and tumor growth in mice xenograft model were evaluated, and the mechanism of its radio-sensitization focusing on mitochondrial function was revealed. Metformin significantly enhanced radio-sensitivity of SCs through its inhibition of the mitochondrial function, as shown by decreased oxygen consumption, decreased mitochondrial membrane potential, and decreased ATP production. Additionally, SCs had a higher ability of mitochondrial respiration than ACs, which may have caused difference of their sensitivity of metformin and irradiation. In conclusion, mitochondrial function might play an important role in the sensitivity of metformin and irradiation, and drugs that target mitochondrial respiration, such as metformin, are promising radio-sensitizers to target CSCs. DOI： 10.1016/j.omto.2021.08.007 PubMed researchmap Using the larvae of caddisfly as a biomonitor to assess the spatial distribution and effective half-life of radiocesium in riverine environments in Fukushima, Japan Reviewed Yuki Matsuo, Kunihiko Nakai, Nozomi Tatsuta, Osamu Inanami, Kumiko Yamamoto, Hazuki Mizukawa, Hiromitsu Nagasaka, Futoshi Mizutani, Youichi Chisaki, Toshiki Aiba, Takashi Ohba, Izumi Watanabe, Hiromi Nabeshi, Taiki Higuchi, Yuki Koga, Hideaki Matsumoto, Kou Nishimuta, Hideki Miyamoto, Tomokazu Haraguchi, Noriko Ryuda, Daisuke Ueno Physics Open   6   100060 - 100060   2021.02 　More details Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV   DOI： 10.1016/j.physo.2021.100060 Radiation-induced abnormal centrosome amplification and mitotic catastrophe in human cervical tumor HeLa cells and murine mammary tumor EMT6 cells Reviewed Masaki Fujimoto, Tomoki Bo, Kumiko Yamamoto, Hironobu Yasui, Tohru Yamamori, Osamu Inanami Journal of Clinical Biochemistry and Nutrition   67 ( 3 )   240 - 247   2020.11 　More details Publishing type：Research paper (scientific journal)   Publisher：The Society for Free Radical Research Japan   DOI： 10.3164/jcbn.19-80 Mitochondrial fission promotes radiation-induced increase in intracellular Ca2+ level leading to mitotic catastrophe in mouse breast cancer EMT6 cells. Reviewed Tomoki Bo, Tohru Yamamori, Kumiko Yamamoto, Masaki Fujimoto, Hironobu Yasui, Osamu Inanami Biochemical and biophysical research communications   522 ( 1 )   144 - 150   2020.01 　More details Language：English   Publishing type：Research paper (scientific journal)   Mitochondrial dynamics are crucial for cellular survival in response to various stresses. Previously, we reported that Drp1 promoted mitochondrial fission after x-irradiation and its inhibition resulted in reduced cellular radiosensitivity and mitotic catastrophe. However, the mechanisms of radiation-induced mitotic catastrophe related to mitochondrial fission remain unclear. In this study, we investigated the involvement of cellular ATP production, ROS generation, and Ca2+ levels in mitotic catastrophe in EMT6 cells. Knockdown of Drp1 and Fis1, which are mitochondrial fission regulators, resulted in elongated mitochondria and significantly attenuated cellular radiosensitivity. Reduced mitochondrial fission mainly decreased mitotic catastrophe rather than necrosis and apoptosis after irradiation. Cellular ATP contents in Drp1 and Fis1 knockdown cells were similar to those in control cells. N-acetylcysteine and 2-glucopyranoside ascorbic acid have no effect on mitotic catastrophe after irradiation. The cellular [Ca2+]i level increased after irradiation, which was completely suppressed by Drp1 and Fis1 inhibition. Furthermore, BAPTA-AM significantly reduced radiation-induced mitotic catastrophe, indicating that cellular Ca2+ is a key mediator of mitotic catastrophe induction after irradiation. These results suggest that mitochondrial fission is associated with radiation-induced mitotic catastrophe via cytosolic Ca2+ regulation. DOI： 10.1016/j.bbrc.2019.11.027 PubMed display all >> To the head of this page.▲ Copyright © TOKYO UNIVERSITY OF AGRICULTURE FOUNDED, All Rights Reserved.