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Contact CORE RESEARCH CLUSTERTOHOKU UNIVERSITY 日本語 Search HOME CORE RESEARCH CLUSTER for MATERIALS SCIENCE ABOUT US RESEARCH PROJECT RESEARCH PROJECT Advanced Energy Materials Project Advanced Electronic Materials Project Biomaterials Project Materials Development Based on Microstructure Control Project High Strength Materials Project Mathematical Science and Computation Evaluation and Sensing Processing Education and Human Resources Development INTERNATIONAL PRESENCE NEWS ＆ EVENTS PUBLICITY NEWS PUBLIC Search TOP ABOUT US RESEARCH PROJECT Advanced Energy Materials Project Advanced Electronic Materials Project Biomaterials Project Materials Development Based on Microstructure Control Project High Strength Materials Project Mathematical Science and Computation Evaluation and Sensing Processing Education and Human Resources Development INTERNATIONAL PRESENCE NEWS ＆ EVENTS Tohok UniversityCreating GlobalExcellence Core Research Cluster for Materials Science Advanced Institute for Materials Research Institute for Materials Research Institute of Multidisciplinary Research for Advanced Materials School of Engineering Department of Metallurgy, Materials Science and Materials Processing Department of Applied Chemistry, Chemical Engineering and Biomolecular Engineering Graduate School of Science and Faculty of Science News and Events NEWS Press release Date uploaded: April 21, 2023 Back to list Structure of Bifidobacterial Enzyme at Room Temperature Observed by X-ray Free Electron Laser - Enzymatic Reaction Mechanism Suggested by Local Structural Changes Press Release 20230410 Summary A joint research group of Sou Iwata, Eriko Nango, and Kensuke Tono has determined the steric structure of the glycolytic enzyme of bifidobacteria at room temperature and found new insights into the reaction mechanism of the enzyme. This research result indicates that obtaining a protein structure close to the physiological environment may provide more useful information for clarifying the enzyme reaction mechanism, and is expected to contribute to the advancement of the industrial use of enzymes through the design of various enzymes with higher functionality. This time, the group succeeded in analyzing the room temperature structure of phosphoketolase, one of the glycolytic enzymes of bifidobacteria, by the continuous femtosecond crystallography (SFX) method at the X-ray free electron laser (XFEL) facility "SACLA". As a result, they found that the small loop-like structure located at the entrance of the active site of phosphoketolase is different from the conventional cryogenic structure. The discussion based on the characteristics of the structure in complex with inhibitors, which was conducted at the same time, provided new insights into the reaction mechanism of phosphoketolase. The phosphoketolase microcrystals needed for the analysis were obtained using a light-induced protein crystallization plate, and this crystallization method was also found to be effective in improving the efficiency of structural analysis at SACLA. This research was published in the online edition of the scientific journal Acta Crystallographica Section D on March 28. (The original press release is in Japanese) https://www.tohoku.ac.jp/japanese/2023/04/press20230410-01-enzyme.html http://www2.tagen.tohoku.ac.jp/lab/news_press/20230410/ Publication details Title: Ambient temperature structure of phosphoketolase from Bifidobacterium longum determined by serial femtosecond X-ray crystallography Authors Kunio Nakata, Tatsuki Kashiwagi, Naoki Kunishima, Hisashi Naitow, Yoshinori Matsuura, Hiroshi Miyano, Toshimi Mizukoshi, Kensuke Tono, Makina Yabashi, Eriko Nango and So Iwata Journal Acta Crystallographica Section D, 79, 290-303, 2023. DOI 10.1107/S2059798323001638 Contact (Research) Professor　Eriko NANGO E-mail: eriko.nango.c4 [at]tohoku.ac.jp Please replace [at] with "@" in the email address. (Press) E-mail: press.tagen [at]grp.tohoku.ac.jp Please replace [at] with "@" in the email address. CORE RESEARCH CLUSTERTOHOKU UNIVERSITY 2-1-1 Katahira, Aoba-ku, Sendai, 980-8577 JAPAN Phone : +81-22-217-5981 Fax : +81-22-217-5311 ABOUT US RESEARCH PROJECT INTERNATIONAL PRESENCE NEWS ＆ EVENTS Contact ACCESS MAP 2-1-1 Katahira, Aoba-ku, Sendai, 980-8577 JAPAN Phone : +81-22-217-5302 Fax : +81-22-217-5311 Copyright © 2019 Tohoku University. All Rights Reserved.